cul1 plasmid Search Results


nf pcmv3 sp n flag pdl1 cd274 cdna sino biological cat  (Sino Biological)
94
Sino Biological nf pcmv3 sp n flag pdl1 cd274 cdna sino biological cat
Nf Pcmv3 Sp N Flag Pdl1 Cd274 Cdna Sino Biological Cat, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nf pcmv3 sp n flag pdl1 cd274 cdna sino biological cat/product/Sino Biological
Average 94 stars, based on 1 article reviews
nf pcmv3 sp n flag pdl1 cd274 cdna sino biological cat - by Bioz Stars, 2026-06
94/100 stars
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gene targeting  (Addgene inc)
92
Addgene inc gene targeting
Gene Targeting, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene targeting/product/Addgene inc
Average 92 stars, based on 1 article reviews
gene targeting - by Bioz Stars, 2026-06
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erα af 1  (Addgene inc)
90
Addgene inc erα af 1
Erα Af 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
erα af 1 - by Bioz Stars, 2026-06
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paper n a pd pcdna3 myc3 cul1 ohta et  (Addgene inc)
93
Addgene inc paper n a pd pcdna3 myc3 cul1 ohta et
Paper N A Pd Pcdna3 Myc3 Cul1 Ohta Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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plasmid pdsp1  (Addgene inc)
93
Addgene inc plasmid pdsp1
Plasmid Pdsp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
plasmid pdsp1 - by Bioz Stars, 2026-06
93/100 stars
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pcmv7 1 3xflag cul1  (Addgene inc)
92
Addgene inc pcmv7 1 3xflag cul1
Pcmv7 1 3xflag Cul1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv7 1 3xflag cul1/product/Addgene inc
Average 92 stars, based on 1 article reviews
pcmv7 1 3xflag cul1 - by Bioz Stars, 2026-06
92/100 stars
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gst smad3 mh2  (Addgene inc)
85
Addgene inc gst smad3 mh2
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Gst Smad3 Mh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst smad3 mh2/product/Addgene inc
Average 85 stars, based on 1 article reviews
gst smad3 mh2 - by Bioz Stars, 2026-06
85/100 stars
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human cul1 expression plasmids  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare human cul1 expression plasmids
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Human Cul1 Expression Plasmids, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cul1 expression plasmids/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
human cul1 expression plasmids - by Bioz Stars, 2026-06
90/100 stars
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human cul1 gene orf cdna clone expression plasmid, n-flag tag  (Sino Biological)
94
Sino Biological human cul1 gene orf cdna clone expression plasmid, n-flag tag
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Human Cul1 Gene Orf Cdna Clone Expression Plasmid, N Flag Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cul1 gene orf cdna clone expression plasmid, n-flag tag/product/Sino Biological
Average 94 stars, based on 1 article reviews
human cul1 gene orf cdna clone expression plasmid, n-flag tag - by Bioz Stars, 2026-06
94/100 stars
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human cul1 gene orf cdna clone expression plasmid c ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human cullin 1
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cul-1 hdr plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR/Cas9 KO Plasmids consists of CUL-1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
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plvx cul1 dn v5 ires zsgreen (plasmid #90440)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Fig. 1. Endogenous Smad3 and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), MH2 (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.

Journal: Development (Cambridge, England)

Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

doi: 10.1242/dev.078345

Figure Lengend Snippet: Fig. 1. Endogenous Smad3 and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), MH2 (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.

Article Snippet: GST-Smad3 MH2 (199-425) and Linker-MH2 (146-425) domains were acquired from Addgene.

Techniques: Size-exclusion Chromatography, Co-Elution Assay, Co-Immunoprecipitation Assay, Pull Down Assay, Staining, Western Blot, Control, Knockdown, Expressing, Software